DIECA (茉莉酸合成途徑抑制劑)
別名：Sodium
diethyldithiocarbamate trihydrate
CAS號：20624-25-3 (C2H5)2NCSSNa
· 3H2O MW 225.31
● Store at RT；Ship at RT
● Specifications
Purity……………………≥98.5%
Appearance……………white
to off-white power or flaky crystal
Solubility……………….. soluble
in water，ethanol，methanol
● 作用機理：
Sodium
diethyldithiocarbamate (DIECA) has been used as a JA biosynthesis inhibitor in
plants and likely inhibits the JA pathway by shunting
13(S)-hydroperoxylinolenic acid to 13-hydroxylinolenic, thereby sharply
reducing the precursor pool leading to cyclization and eventual synthesis of JA
(Farmer, Caldelari, Pearce, Walker-Simmons, & Ryan, 1994).
● 母液配制方法：
1. 20 ml 0.2 M DIECA配制（水溶解）：
稱取0.9 g粉末置于50 ml尖底螺口離心管中，擰緊管蓋，加入蒸餾水定容至20 ml，漩渦攪拌至徹底混勻，溶液應該為接近澄清透明，0.22 μm水系濾膜抽濾后-20℃貯存。
注意：DIECA溶液配制時不能開口攪拌混勻，因為溶液吸收空氣中的二氧化碳，會使溶液變渾濁，無法完全溶解。
2. 20 ml 0.2 M DIECA配制（DMSO溶解）：
使用DMSO可以配制成完全澄清透明的溶液，溶解方法同1。0.22 μm有機系濾膜抽濾后-20℃貯存。
● 文獻：
1. Farmer, E. E., Caldelari, D.,
Pearce, G., Walker-Simmons, M. K., & Ryan, C. A. (1994). Diethyldithiocarbamic
acid inhibits the octadecanoid signaling pathway for the wound induction of
proteinase inhibitors in tomato leaves. Plant Physiology, 106(1), 337– 342.
2. Yinghui Li,Lina Qiu,Qiang
Zhang,Xiangxi Zhuansun,Huifang Li,Xin Chen,Tamar
Krugman,Qixin Sun,Chaojie Xie.
（2020）. Exogenous sodium
diethyldithiocarbamate, a Jasmonic acid biosynthesis inhibitor, induced
resistance to powdery mildew in wheat. Plant
Direct. Volume 4, Issue4, e00212.
DIECA產品發(fā)表文章
1. [2020 IF=2.477] The Jasmonic Acid
Pathway Positively Regulates the Polyphenol Oxidase-Based Defense against Tea
Geometrid Caterpillars in the Tea Plant (Camellia
sinensis).
Author: Zhang, Jin; Zhang, Xin;
Ye, Meng; Li, Xi-Wang, XiaoLing Sun
Journal: Journal of Chemical Ecology. 2021:
46: 308-316
Institution： Tea Research Institute, Chinese Academy of Agricultural
Sciences
Paper link：https://doi.org/10.1007/s10886-020-01158-6
2. [2021 IF=12.085] Jasmonate activates a
CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic
genes and fruit coloration in orange (Citrus sinensis).
Author: Pengtao Yue, Zhenghua
Jiang, Quan Sun, Ranran Wei, Yingzi Yin, Zongzhou Xie, Robert M. Larkin, Junli
Ye , Lijun Chai and Xiuxin Deng
Journal: Plant Cell. 2023: 35: 1167–1185
Institution： Huazhong Agricultural University
Paper link： https://academic.oup.com/plcell/article/35/4/1167/6931829?login=false
3. [2022 IF=3.1] Jasmonate
Promotes Ester Aroma Biosynthesis during Nanguo Pears Storage.
Author: Liyong Qi, Chuhan Li,
Jianan Sun, Weiting Liu, Yueming Yang, Xiaojing Li,Hongjian Li, Yuqi Du , Islam
Mostafa and Zepeng Yin
Journal: horticulturae. 2024, 10, 329.
Institution： College of Horticulture, Shenyang Agricultural University
Paper link：https://doi.org/10.3390/horticulturae10040329