Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.
貨號(hào)：C0203.0050
規(guī)格：50升
價(jià)格：500元
產(chǎn)地：Duchefa
N6 Medium(CHU Medium) not including Vitamins
Total concentration Micro and Macro elements not including vitamins: 3952.53 mg/l
|
Macro Elements
|
mg/l
|
mM
|
|
CaCl2
|
125.33
|
1.13
|
|
KH2PO4
|
400.00
|
2.94
|
|
KNO3
|
2830.00
|
27.99
|
|
MgSO4
|
90.27
|
0.75
|
|
(NH4)2SO4
|
463.00
|
3.50
|
|
Micro Elements
|
mg/l
|
μM
|
|
FeNaEDTA
|
36.70
|
100.00
|
|
H3BO3
|
1.60
|
25.88
|
|
KI
|
0.80
|
4.81
|
|
MnSO4.H2O
|
3.33
|
19.70
|
|
ZnSO4.7H2O
|
1.50
|
5.22
|
|
|
|
|
Chu C.C, The N6 medium and its application to anther culture of -cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).
Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen -sources. Scienta Sinic., 18, 659 (1975).